Kinetics and template-dependency of ribonucleic acid synthesis by bacterial ribonucleic acid polymerase
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چکیده
منابع مشابه
Enzymatic Synthesis of Ribonucleic Acid. I. Preparation of Ribonucleic Acid Polymerase from Extracts of Micrococcus Lysodeikticus.
The problem of complex polynucleotide synthesis has received considerable attention from numerous investigators (l-3). Grunberg-Manago and Ochoa (4) and Kornberg, Lehman, and Simms (5) first successfully demonstrated the polymerization in vitro of riboand deoxynucleotides with cell-free extracts from microorganisms. Subsequent studies on the synthesis of ribonucleic acid and deoxyribonucleic ac...
متن کاملThe Enzymatic Synthesis of Ribonucleic Acid. v. the Interaction of Ribonucleic Acid Polymerase with Nucleic Acids.
Numerous reports have been published on the properties and reaction mechanism of the highly purified enzyme preparations that catalyze the deoxyribonucleic acid-directed synthesis of ribonucleic acid (l-6). The various RNA polymerases tudied, although differing in some respects, show a remarkable similarity in their cofactor requirements, reaction kinetics, physical properties, and facility to ...
متن کاملTemplate Specificity of Oligonucleotides for Ribonucleic Acid Polymerase.
Recently attempts have been made to d&m: chemically the templates used as substrates in bacterial ribonucleic acid polymerase systems and to analyze the RX.1 products of such systems (l-9). Enzymatically synthesized polydeosynucleotides (2, 3, 6, 8) and polyribonucleotides (5, 7, 9, 10) permitted studies of the mechanism involved in the polyribonucleotide synthesis. It has been shown that an RX...
متن کاملEnzymatic Synthesis of Ribonucleic Acid 1. PREPARATION OF RIBONUCLEIC ACID POLYMERASE FROM EXTRACTS OF MICROCOCCUS LYSODEIKTICUP
The problem of complex polynucleotide synthesis has received considerable attention from numerous investigators (l-3). Grunberg-Manago and Ochoa (4) and Kornberg, Lehman, and Simms (5) first successfully demonstrated the polymerization in vitro of riboand deoxynucleotides with cell-free extracts from microorganisms. Subsequent studies on the synthesis of ribonucleic acid and deoxyribonucleic ac...
متن کاملAzotobacter vinehndii Ribonucleic Acid Polymerase
RNA synthesis by the Azofobacter vinelandii RNA polymerase is initiated with the incorporation of a ribonucleoside triphosphate to form the 5’ terminus of the chain. The RNA chains synthesized in vifro are preferentially initiated by purine ribonucleoside triphosphates. The ratio of guanosine triphosphate to ATP termini is determined by the DNA used to direct the synthesis of RNA. When denature...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1978
ISSN: 0264-6021
DOI: 10.1042/bj1690421